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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
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Pseudogenes as an alternative source of natural antisense transcripts.

Enrique M Muro1, Miguel A Andrade-Navarro

  • 1Max-Delbrück Center for Molecular Medicine, Robert Rössle Str, 10, 13125 Berlin, Germany. enrique.muro@mdc-berlin.de

BMC Evolutionary Biology
|November 5, 2010
PubMed
Summary

Pseudogenes can generate trans-antisense transcripts (trans-NATs) that regulate parental genes. Analysis of expressed sequence tags revealed conserved regions in pseudogene-derived trans-NATs, suggesting a potential functional role.

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Area of Science:

  • Genomics
  • RNA Biology
  • Evolutionary Biology

Background:

  • Naturally occurring antisense transcripts (NATs) are non-coding RNAs that can regulate gene activity through complementary binding.
  • Trans-NATs, which regulate genes other than their own, evolve more readily than cis-NATs.
  • The evolution of trans-NATs requires substantial complementarity to their target genes.

Purpose of the Study:

  • To investigate pseudogene formation as an evolutionary mechanism for generating trans-NATs.
  • To analyze the characteristics of trans-NATs originating from human pseudogenes.

Main Methods:

  • Analysis of the expressed sequence tag (EST) database to identify human pseudogenes with trans-NATs.
  • Examination of mutation patterns in pseudogene-derived trans-NATs to assess their functional conservation.

Main Results:

  • Identified human pseudogenes containing trans-NATs complementary to their parental genes.
  • Observed non-random mutation distribution in trans-NATs, with higher similarity to parental genes near the 3' end.
  • This suggests that these trans-NATs are not entirely non-functional.

Conclusions:

  • Pseudogene formation is a plausible mechanism for generating trans-NATs.
  • The findings provide evidence supporting a potential functional relationship between pseudogene-derived trans-NATs and their parental genes.
  • Highlights the significance of genetic material duplication in the evolution of non-coding RNAs.