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Related Experiment Video

Updated: Jun 7, 2026

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Isolation and Culture Expansion of Tumor-specific Endothelial Cells

Published on: October 14, 2015

Stem cells in tumor angiogenesis.

Shentong Fang¹, Petri Salven

¹Molecular Cancer Biology Program & Department of Pathology, University of Helsinki, Helsinki, Finland.

Journal of Molecular and Cellular Cardiology

|November 5, 2010

Summary

Tumor angiogenesis relies on diverse stem cells, not just endothelial progenitors (EPCs). Hematopoietic stem cells (HSCs) and other vascular stem cells play key roles in cancer growth.

Area of Science:

Stem cell biology
Cancer research
Angiogenesis

Background:

Tumor angiogenesis was thought to require bone marrow (BM)-derived endothelial progenitors (EPCs).
Recent studies challenge the necessity of EPCs, suggesting they may be artifactual.
Tumor-infiltrating hematopoietic cells derived from BM hematopoietic stem cells (HSCs) are implicated in tumor angiogenesis.

Purpose of the Study:

To identify critical hematopoietic cell subpopulations involved in tumor angiogenesis.
To elucidate the proangiogenic functions and mechanisms of these cells.
To explore the potential of undiscovered vascular endothelial stem cells (VESCs) for therapeutic strategies.

Main Methods:

Review of recent studies on stem cells and tumor angiogenesis.

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Analysis of the roles of hematopoietic stem cells (HSCs) and other stem/progenitor cells.

Hypothesizing the existence and function of vascular endothelial stem cells (VESCs).

Main Results:

Endothelial progenitors (EPCs) are not essential for tumor angiogenesis.
Hematopoietic stem cells (HSCs) and their derivatives contribute to tumor angiogenesis via paracrine signaling and extracellular matrix remodeling.
Other stem and progenitor cell subsets, including pericytes and mesenchymal/stromal cells, may also contribute to neovasculature.

Conclusions:

Tumor angiogenesis involves a broader range of stem and progenitor cells than previously understood.
Understanding these cell populations offers potential therapeutic targets for blocking cancer growth.
The hypothesis of a tissue-specific vascular endothelial stem cell (VESC) presents a novel avenue for anti-angiogenic therapies.