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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
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Published on: May 20, 2013

Targeted three-dimensional liquid chromatography: a versatile tool for quantitative trace analysis in complex

Scott W Simpkins1, Jeremy W Bedard, Stephen R Groskreutz

  • 1Gustavus Adolphus College, 800 West College Avenue, Saint Peter, MN 56082, USA.

Journal of Chromatography. A
|November 5, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a three-dimensional High-Performance Liquid Chromatography (3D HPLC) system for targeted analysis. This advanced method simplifies complex sample analysis and speeds up method development for environmental and biological samples.

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Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Targeted multidimensional liquid chromatography (MDLC) is established for analyzing low-concentration analytes in complex samples.
  • Conventional MDLC primarily uses two dimensions, with limited exploration of a third dimension.
  • Method development for complex samples is often simplified by increased resolving power from additional separation dimensions.

Purpose of the Study:

  • To construct and evaluate a three-dimensional HPLC (3D HPLC) system for targeted analysis.
  • To demonstrate the benefits of a third separation dimension in MDLC.
  • To reduce the complexity and time associated with method development for analyzing low-concentration analytes in diverse matrices.

Main Methods:

  • Development of a 3D HPLC system utilizing three distinct reversed-phase columns.
  • Application of statistical peak overlap theory and reversed-phase selectivity models for simulation.
  • Experimental validation using urban wastewater, human urine, and river water samples.
  • Quantitative comparison with conventional one-dimensional LC-MS/MS.

Main Results:

  • Simulations predicted significant benefits of a third dimension in MDLC.
  • Experimental methods achieved excellent separation of target compounds from complex matrices.
  • A single set of separation conditions proved effective across various sample types.
  • Targeted 3D HPLC with UV detection demonstrated quantitative accuracy and low part-per-trillion detection limits.

Conclusions:

  • The developed 3D HPLC system offers a powerful tool for targeted analysis.
  • This approach significantly streamlines method development for complex mixtures.
  • 3D HPLC shows promise as a superior alternative to 2D separations for rapid and robust quantitation.