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In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Specified species in gingival crevicular fluid predict bacterial diversity
Sirkka Asikainen1, Başak Doğan, Zeynep Turgut
1Section of Oral Microbiology, Institute of Odontology, Umeå University, Umeå, Sweden. sirkka.asikainen@odont.umu.se
Plos One
|November 5, 2010
Summary
Gingival crevicular fluid (GCF) analysis reveals diverse bacterial species, with Gram-negative anaerobes predicting diversity and distinguishing GCF from paper point samples in periodontitis patients.
Area of Science:
- Microbiology
- Periodontology
- Molecular Biology
Background:
- Gingival crevicular fluid (GCF) analysis offers insights into unattached subgingival bacteria.
- This study is the first to identify bacterial species directly within GCF samples.
Purpose of the Study:
- To determine bacterial species diversity in GCF of periodontitis patients.
- To identify bacterial and host-associated factors influencing this diversity.
- To compare GCF bacterial profiles with subgingival paper point (PP) samples.
Main Methods:
- DNA extraction from GCF and PP samples.
- PCR amplification of 16S rRNA genes.
- DNA-DNA hybridization using a microarray for over 300 bacterial species.
- Multivariate data analysis using Projection to Latent Structures (PLS) and Orthogonal Partial Least Squares (OPLS).
Main Results:
- Detected 133 species from 41 genera and 8 phyla across GCF and PP samples.
- PLS regression identified specific bacterial genera (e.g., Campylobacter, Porphyromonas) positively correlated with GCF species diversity.
- OPLS discriminant analysis highlighted genera like Campylobacter, Prevotella, and Fusobacterium as key in distinguishing GCF samples.
Conclusions:
- Gram-negative anaerobes in mature subgingival biofilms are key predictors of GCF bacterial diversity.
- These bacteria also differentiate GCF samples from PP samples.
- GCF analysis presents novel opportunities for studying subgingival biofilm dynamics.
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