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Updated: Jun 7, 2026
Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein
Published on: January 28, 2013
Retinol fluorescence in lecithin/n-butanol/water aggregates: a new improvement for its analysis in cosmetics without
M P San Andrés1, S Vera, M Torre
1Department of Analytical Chemistry and Chemical Engineering, University of Alcalá, 28871, Alcalá de Henares, Madrid, Spain. mpaz.sanandres@uah.es
Abstract:
The possibilities of different media formed by lecithin/n-butanol (n-BuOH)/water ternary mixtures for the analysis of all-trans-retinol by fluorescence have been studied. Fluorescence intensity of retinol increases in the presence of different types of aggregates formed in these media. Analytical features are good, the detection limit and quantification limit have micrograms per liter levels, and the linear range and sensitivity are appropriate to determine retinol in cosmetic samples. The analysis of retinol in anti-wrinkle creams can be achieved directly without any pretreatment of the sample. The vesicles built up from a biocompatible surfactant (lecithin) in aqueous solution with a low amount of n-BuOH permit an appropriated media for a simple, rapid, and sensitive analytical method. This method has a linear range between 64.1 and 800 μg L(-1), a sensitivity of 202.3 L mg(-1), and a low detection and quantification limit at 19.2 and 64.1 μg L(-1), respectively.
