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[Determination of berberine chloride in mouse plasma after intragastric administration of Lianxiang microemulsion]
Wen-Biao Lu1, Wei-Wen Chen, Ru-Liu Li
1Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405, China. vip.luwbl@gzhtcm.edu.cn
Objective:
To determine berberine chloride in mouse plasma for the investigation of the bioavailability of Lianxiang microemulsion in mouse.
Methods:
After single dose ig administration,the plasma samples were taken at different time,respectively, for the determination of berberine chloride by HPLC; And the relative bioavailability of Lianxiang microemulsion vs Lianxiang emulsion (reference formulation) was calculated.
Results:
Berberine chloride was separated well from endogenous foreign substances. The calibration curve of berberine chloride was liner over the range of 10.4-156 microg/L (r = 0.9991) and the method recovery was within 89.6%-94.7%. The intraday RSD or the inter-day RSD < or = 12.9%. After single dose,the AUC0-->24h of the microemulsion and the emulsion were (688.3 +/- 123.7) and (371.4 +/- 68.4) microg x h/L,respectively; And the relative bioavailability of the microemulsion vs the emulsion was (193.1 +/- 63.2)%.
Conclusion:
The validated HPLC method is suitable for the determination of berberine chloride in mouse plasma; And microemulsification can clearly promote the bioavailability in mouse of berberine chloride in Lianxiang prescription.