Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Antianginal Drugs: Nitrates and β-Blockers01:16

Antianginal Drugs: Nitrates and β-Blockers

In cardiovascular health, antianginal drugs combat angina pectoris — a condition marked by chest pain owing to diminished blood flow to the heart.
Organic nitrates,  such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow. Administered...
Antihypertensive Drugs: Vasodilators01:23

Antihypertensive Drugs: Vasodilators

Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
Types of Signaling Molecules01:32

Types of Signaling Molecules

In multicellular organisms, many molecules transmit signals between cells to pass information. These signals vary in complexity and include small peptides, nucleotides, steroids, fatty acid derivatives, and dissolved gases such as nitric oxide. Some signaling molecules diffuse through the plasma membrane to act locally between neighboring cells or travel long distances. Others remain attached to the cell surface, transmitting information to other cells only when they make contact. In some...
Paracrine Signaling01:21

Paracrine Signaling

Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Hexokinase controls platelet activation and hemostasis.

Platelets·2026
Same author

GPVI deficiency reduces clot size and murine thrombosis in normoglycaemic but not hyperglycemic conditions.

Platelets·2026
Same author

Phenotyping of human platelets in response to platelet agonists and inhibitors using multiparameter flow cytometry and unbiased high-dimensional analysis.

Research and practice in thrombosis and haemostasis·2025
Same author

Extracellular Vesicles From a Model of Melanoma Cancer-Associated Fibroblasts Induce Changes in Brain Microvascular Cells Consistent With Pre-Metastatic Niche Priming.

Journal of extracellular biology·2025
Same author

The NLRP3 inflammasome in platelets - form, functions, and future of the complex.

Platelets·2025
Same author

The immune receptor FcRγ-chain mediates CD36-induced platelet activation and thrombosis by oxidized low-density lipoproteins.

Blood advances·2025
Same journal

Chemiluminescence resonance energy transfer-based method to investigate the platelet surface molecule in acute myocardial infarction.

Platelets·2026
Same journal

Nanobodies to GPVI as alternative reagents for platelet spreading.

Platelets·2026
Same journal

Neural network reveals platelet age from fluorescence microscopy images.

Platelets·2026
Same journal

Sixty years of research into ancestry differences in platelet function.

Platelets·2026
Same journal

Platelet-rich plasma concentrations regulate MSCs osteogenesis via MAPK/PI3K-AKT pathways to mitigate inflammatory bone loss.

Platelets·2026
See all related articles

Related Experiment Video

Updated: Jun 7, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Nitric oxide at a glance.

Khalid M Naseem1, Wayne Roberts

  • 1Hull York Medical School, University of Hull, Hull, Yorkshire, UK. khalid.naseem@hyms.ac.uk

Platelets
|November 6, 2010
PubMed
Summary
This summary is machine-generated.

Nitric oxide (NO) and prostacyclin (PGI2) from healthy blood vessels prevent excessive platelet activation. This review explores NO

More Related Videos

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Related Experiment Videos

Last Updated: Jun 7, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Analytical Techniques for Assaying Nitric Oxide Bioactivity
11:28

Analytical Techniques for Assaying Nitric Oxide Bioactivity

Published on: June 18, 2012

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

Area of Science:

  • Vascular Biology
  • Hematology
  • Biochemistry

Background:

  • Platelet activation is crucial for hemostasis but requires regulation to prevent thrombosis.
  • Endothelial cells produce nitric oxide (NO) and prostacyclin (PGI2) to inhibit platelet activation.
  • Dysregulation of these pathways contributes to cardiovascular diseases.

Purpose of the Study:

  • To review the current understanding of nitric oxide (NO)-mediated regulation of platelet function.
  • To highlight unresolved questions in NO-platelet signaling.

Main Methods:

  • Literature review of existing research on NO signaling in platelets.
  • Analysis of molecular mechanisms involved in NO-induced platelet modulation.
  • Synthesis of findings on the role of soluble guanylyl cyclase (sGC) and cyclic nucleotides.

Main Results:

  • Nitric oxide (NO) primarily signals through soluble guanylyl cyclase (sGC), leading to increased cyclic GMP (cGMP).
  • Elevated cGMP levels inhibit key platelet activation pathways, including aggregation and adhesion.
  • NO also influences other platelet functions, though mechanisms are less defined.

Conclusions:

  • Nitric oxide (NO) plays a critical inhibitory role in platelet activation via cGMP-dependent pathways.
  • Further research is needed to fully elucidate the complex NO-mediated signaling network in platelets.
  • Understanding these pathways is vital for developing novel antiplatelet therapies.