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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: Jun 7, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
10:28

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Published on: June 30, 2016

Tissue optical immersion clearing.

Elina A Genina1, Alexey N Bashkatov, Valery V Tuchin

  • 1Research-Educational Institute of Optics and Biophotonics, Saratov State University, 410012 Saratov, Russia.

Expert Review of Medical Devices
|November 6, 2010
PubMed
Summary

The optical immersion method enhances tissue and blood optical clearing by matching refractive indices. This technique unlocks hidden information for advanced imaging and sensing applications.

Area of Science:

  • Biophotonics and Biomedical Optics
  • Tissue Optics and Imaging

Background:

  • Scattering by cellular structures and extracellular matrix limits light penetration in biological tissues.
  • Existing optical clearing methods face challenges in achieving comprehensive refractive index matching.
  • Hidden information in spectroscopic and imaging data is often obscured by scattering.

Purpose of the Study:

  • To discuss the optical immersion method for refractive index matching in tissues and blood.
  • To analyze optical clearing of various biological samples using exogenous agents.
  • To explore the potential for enhanced information retrieval from advanced optical techniques.

Main Methods:

  • Refractive index matching of scatterers (collagen, elastin, cells) with ground material (interstitial fluid, cytoplasm).

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Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging

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Related Experiment Videos

Last Updated: Jun 7, 2026

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY
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Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Published on: June 30, 2016

Optical Clearing and Imaging of Immunolabeled Kidney Tissue
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  • Application of exogenous optical clearing agents.
  • Analysis of spectroscopic, polarization, microscopy, tomography, and nonlinear spectroscopy techniques.

    • Main Results:

    • Optical clearing of fibrous and cell-structured tissues and blood is achieved.
    • Improved signal-to-noise ratio and information content from optical measurements.
    • Demonstrated potential for enhanced imaging depth and contrast.

    Conclusions:

    • The optical immersion method significantly improves optical clearing of biological samples.
    • This technique enables access to previously hidden information for diagnostic and research purposes.
    • Applications span glucose sensing, drug delivery monitoring, and advanced laser-tissue interactions.