Design and use of constitutively active STAT5 constructs
Related Experiment Video
Updated: Jun 7, 2026

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
Published on: October 9, 2016
1Center for Immunology, Masonic Cancer Center, University of Minnesota, Minneapolis, USA.
Abstract:
The transcription factor Signal Tranducer and Activator of Transcription 5 (STAT5) plays an important role in numerous biological processes including, but not limited to, (i) homeostasis of hematopoietic stem cells, (ii) development of essentially all blood cell lineages, (iii) growth hormone effects, (iv) differentiation of mammary epithelium, and (v) central nervous system control of metabolism. Two key tools for deciphering STAT5 biology have involved the use of mice in which the Stat5a and Stat5b genes can be conditionally deleted (Stat5(FL/FL) mice) and the development of systems in which STAT5a or STAT5b is rendered constitutively active. In this chapter, the distinct mechanisms that have been developed to render STAT5 constitutively active and their use in probing biological processes are discussed.
Related Concept Videos
Constitutive and Regulated Gene Expression
The JAK-STAT Signaling Pathway
Cooperative Binding of Transcription Regulators
Cooperative Binding of Transcription Regulators
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein.
