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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: Jun 7, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

ASPicDB: a database of annotated transcript and protein variants generated by alternative splicing.

Pier L Martelli1, Mattia D'Antonio, Paola Bonizzoni

  • 1Biocomputing Group, University of Bologna, Bologna 40126, Italy.

Nucleic Acids Research
|November 6, 2010
PubMed
Summary

ASPicDB offers a comprehensive resource for human protein variants arising from alternative splicing. This updated database provides detailed functional annotations to aid research into transcriptome and proteome diversity.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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Last Updated: Jun 7, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Alternative splicing significantly expands transcriptome and proteome diversity in vertebrates.
  • The functional relevance of numerous alternative transcripts and proteins remains under investigation.

Purpose of the Study:

  • To present an updated release of ASPicDB, a specialized annotation resource for human protein variants generated by alternative splicing.
  • To provide detailed functional annotations for these variants using advanced machine learning tools.

Main Methods:

  • Extensive annotation of 256,939 protein variants from 17,191 multi-exon human genes.
  • Utilized machine learning for predicting protein type, localization, PFAM domains, signal peptides, and other structural features.
  • Incorporated CAGE-tags and polyA signals for identifying transcription initiation and termination sites to specifically select full-length variants.

Main Results:

  • ASPicDB now includes comprehensive annotations for human protein variants from alternative splicing.
  • The database provides detailed information on protein features, including domains, localization, and post-translational modification sites.
  • New features allow specific selection of full-length variants based on transcription start and end site annotations.

Conclusions:

  • ASPicDB serves as a unique resource for exploring human protein variants generated by alternative splicing.
  • The database facilitates the study of functional diversity arising from alternative splicing events.
  • Enhanced retrieval capabilities enable researchers to select specific datasets based on desired variant features.