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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jun 7, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Morphological resonances for multicomponent immunoassays.

W B Whitten, M J Shapiro, J M Ramsey

|November 6, 2010

Summary

This summary is machine-generated.

A novel immunoassay technique uses optical-resonance size discrimination to detect multiple microorganism species simultaneously. This method identifies stained microorganisms by the antibodies they bind to, enabling single-step analysis.

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Last Updated: Jun 7, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

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Published on: November 10, 2016

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

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Published on: July 14, 2023

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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

Area of Science:

Biotechnology
Analytical Chemistry
Microbiology

Background:

Accurate identification of microorganisms is crucial for diagnostics and research.
Current methods for detecting multiple species can be time-consuming and complex.

Purpose of the Study:

To develop a single, efficient immunoassay for detecting and identifying multiple microorganism species.
To leverage optical-resonance size discrimination for enhanced specificity and speed.

Main Methods:

Development of an immunoassay utilizing microspheres for size discrimination.
Staining of microorganisms to facilitate antibody binding.
Application of optical-resonance technology to identify microspheres bound to specific antibodies and thus, microorganisms.

Main Results:

Successful detection and identification of multiple microorganism species within a single assay.
Demonstration of optical-resonance size discrimination as a viable method for immunoassay signal generation.
High specificity in identifying microorganisms based on antibody-antigen interactions.

Conclusions:

The described immunoassay technique offers a rapid and multiplexed approach for microorganism detection.
This method has potential applications in clinical diagnostics, food safety, and environmental monitoring.
Optical-resonance size discrimination provides a powerful tool for advanced immunoassay development.