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Formulation of a dimensionless overlap fraction to account for spectrally distributed interactions in fluorescence
Applied Optics
|November 6, 2010
Abstract:
An overlap term is used in fluorescence theory to account for the spectrally distributed interaction between laser radiation and molecular transitions. We present a dimensionless overlap fraction formulation. Compared with the more common dimensional overlap term [in units of inverse wave number (1/cm(-1))], this form of expression of the interaction between a laser and an absorption transition has a much more practical interpretation and simplifies the equations that describe fluorescence measurements.
