Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Role of Hematopoietic Growth Factors

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Factors Affecting Erythropoiesis

Factors Affecting Erythropoiesis

The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...

Structure and Function of Platelets

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Intact Contextual Cueing for Search in Realistic Scenes with Simulated Central or Peripheral Vision Loss.

Translational vision science & technology·2020

Same author

Hydrolysis of Corncob Hemicellulose by Solid Acid Sulfated Zirconia and Its Evaluation in Xylitol Production.

Applied biochemistry and biotechnology·2020

Same author

One-Step Electrodeposition of Silver Nanostructures on 2D/3D Metal-Organic Framework ZIF-67: Comparison and Application in Electrochemical Detection of Hydrogen Peroxide.

ACS applied materials & interfaces·2020

Same author

Chimeric Antigen Receptor Designed to Prevent Ubiquitination and Downregulation Showed Durable Antitumor Efficacy.

Immunity·2020

Same author

Prevalence and antimicrobial resistance of retail-meat-borne Salmonella in southern China during the years 2009-2016: The diversity of contamination and the resistance evolution of multidrug-resistant isolates.

International journal of food microbiology·2020

Same author

The deubiquitinase USP44 promotes Treg function during inflammation by preventing FOXP3 degradation.

EMBO reports·2020

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Thrombopoietin factors.

¹Department of Hematology, Amgen, Inc., Thousand Oaks, CA 91320, USA. pwei@amgen.com

Cancer Treatment and Research

|November 6, 2010

Summary

This summary is machine-generated.

Platelet generation involves megakaryopoiesis and thrombopoiesis. Thrombopoietin (TPO) mimetics like romiplostim and eltrombopag are successful treatments for thrombocytopenia, addressing a key medical need.

More Related Videos

Extracellular Vesicle Tissue Factor Activity Assay

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

Related Experiment Videos

Last Updated: Jun 7, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Extracellular Vesicle Tissue Factor Activity Assay

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Published on: September 9, 2012

Area of Science:

Hematology
Molecular Biology
Pharmacology

Background:

Severe thrombocytopenia poses significant morbidity and mortality risks.
Platelet generation relies on megakaryopoiesis and thrombopoiesis.
Thrombopoietin (TPO) is the primary regulator of these processes.

Purpose of the Study:

To review scientific advancements in thrombopoietic factors over the past two decades.
To focus on the biology, physiology, and pharmacology of TPO and its receptor, c-Mpl.
To discuss the development and success of TPO mimetics.

Main Methods:

Review of scientific literature and clinical trial data.
Analysis of preclinical models for thrombocytopenia treatment.
Examination of TPO biology, c-Mpl receptor interactions, and TPO mimetic pharmacology.

Main Results:

TPO mimetics romiplostim and eltrombopag are approved for chronic immune thrombocytopenic purpura (ITP).
These therapeutics represent successful strategies for managing thrombocytopenia.
Significant progress has been made in understanding TPO-mediated platelet production.

Conclusions:

TPO and its mimetics are crucial for managing thrombocytopenia.
Further research into thrombopoietic factors continues to yield therapeutic advancements.
The development of TPO mimetics has transformed ITP treatment.