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Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

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Published on: September 19, 2022

Intracellular delivery of nanoparticles with CPPs.

Rupa Sawant¹, Vladimir Torchilin

¹Research Associate Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 6, 2010

Summary

This summary is machine-generated.

Cell-penetrating peptides (CPPs) enhance intracellular delivery via nanocarriers. This study details methods for attaching CPPs, like TATp, to nanocarriers and introduces a "smart" nanocarrier with a hidden CPP function for targeted delivery.

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Area of Science:

Biotechnology
Nanomedicine
Drug Delivery

Background:

Cell-penetrating peptides (CPPs) facilitate intracellular delivery of diverse cargoes.
CPPs, particularly TATp, are widely employed for in vitro and in vivo applications.
Attaching CPPs to nanocarriers involves covalent or noncovalent strategies.

Purpose of the Study:

To describe methods for conjugating CPPs, such as TATp, onto nanocarrier surfaces.
To detail the loading of drugs or DNA into CPP-modified nanocarriers.
To present a novel "smart" nanocarrier design with a concealed CPP function to improve targeting.

Main Methods:

Covalent and noncovalent conjugation techniques for attaching TATp to liposomes and micelles.
Loading of therapeutic payloads (drugs, DNA) into functionalized nanocarriers.
In vitro and in vivo characterization of CPP-modified nanocarriers.

Main Results:

Successful conjugation of TATp to nanocarrier surfaces was achieved.
CPP-modified nanocarriers demonstrated efficient cargo loading and intracellular delivery.
A "smart" nanocarrier system with a hidden TATp function was developed, showing potential for targeted delivery.

Conclusions:

Various methods exist for CPP conjugation to nanocarriers for enhanced intracellular delivery.
"Smart" nanocarriers with hidden CPPs offer a strategy to overcome non-specific distribution issues.
This work provides a foundation for developing targeted nanomedicines using CPPs.