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Updated: Jun 7, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

PAIR technology: exon-specific RNA-binding protein isolation in live cells.

Thomas J Bell1, Emelía Eiríksdóttir, Ulo Langel

  • 1Department of Pharmacology and Psychiatry, University of Pennsylvania Medical Center, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 6, 2010
PubMed
Summary

Identifying RNA-binding proteins (RBPs) is challenging. Peptide nucleic acid (PNA)-assisted identification of RBPs (PAIR) technology enables RBP isolation under in vivo conditions, offering greater specificity for mRNA targets.

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Published on: December 2, 2009

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biochemistry

Background:

  • RNA-binding proteins (RBPs) are crucial for gene expression control.
  • Current methods for RBP isolation have limitations, including non-physiological conditions.
  • Investigating RBP-RNA interactions is essential for understanding gene regulation.

Purpose of the Study:

  • To review a novel technology for identifying RNA-binding proteins (RBPs).
  • To highlight the advantages of peptide nucleic acid (PNA)-assisted identification of RBPs (PAIR) technology.
  • To demonstrate PAIR technology's ability to isolate RBP-RNA interactions under in vivo conditions.

Main Methods:

  • Review of peptide nucleic acid (PNA)-assisted identification of RBPs (PAIR) technology.
  • Comparison of PAIR technology with traditional in vitro biochemical approaches.
  • Application of PAIR technology for isolating splice variant-specific and mRNA region-specific RBP complexes.

Main Results:

  • PAIR technology allows RBP isolation under in vivo conditions, overcoming limitations of in vitro methods.
  • PAIR technology offers high mRNA specificity, isolating RBPs in an exon-specific manner.
  • The technology enables targeted isolation of RBP complexes from specific mRNA regions (5-UTR and 3-UTR).

Conclusions:

  • PAIR technology provides a significant advancement for identifying RBPs and their interactions.
  • This method facilitates the study of RBP roles in gene regulation under physiological conditions.
  • PAIR technology enables precise investigation of splice variant-specific RBP binding.