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Related Experiment Video

Updated: Jun 7, 2026

Cecal Ligation Puncture Procedure

Cecal Ligation Puncture Procedure

Published on: May 7, 2011

Cecal ligation and puncture.

Alex G Cuenca¹, Matthew J Delano, Kindra M Kelly-Scumpia

¹University of Florida College of Medicine, Gainesville, Florida, USA.

Current Protocols in Immunology

|November 6, 2010

Summary

This summary is machine-generated.

This study details a novel multifactorial model for bacterial sepsis. By ligating the cecum, it creates ischemic tissue and polymicrobial infection, differentiating it from other sepsis models.

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Last Updated: Jun 7, 2026

Cecal Ligation Puncture Procedure

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Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Published on: December 9, 2022

Area of Science:

Microbiology
Surgical Pathology
Infectious Disease

Background:

The cecum harbors a dense, diverse microbial population, including anaerobic, facultative aerobic, and aerobic bacteria.
Gram-negative and Gram-positive bacteria constitute the cecal flora, contributing to its complex microbial environment.

Purpose of the Study:

To describe a novel multifactorial model for inducing bacterial sepsis.
To differentiate this model from existing bacterial sepsis models through its unique combination of ischemia and infection.

Main Methods:

The described procedure involves the ligation of the cecum.
This ligation induces a state of ischemic/necrotic tissue within the cecum.

Main Results:

The cecal ligation results in both ischemic/necrotic tissue and a polymicrobial infection.
This combined insult creates a unique pathological environment.

Conclusions:

The cecal ligation model offers a distinct approach to studying bacterial sepsis.
This model's combination of ischemic tissue and polymicrobial infection distinguishes it from simpler models like bacteremia or abscess formation.