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Related Concept Videos

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...

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Related Experiment Video

Updated: Jun 7, 2026

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Measuring mast cell mediator release.

Hye Sun Kuehn1, Madeleine Radinger, Alasdair M Gilfillan

  • 1Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.

Current Protocols in Immunology
|November 6, 2010
PubMed
Summary
This summary is machine-generated.

This study details methods to measure mast cell activation, crucial for understanding allergic diseases like anaphylaxis and atopy. These techniques aid in discovering new therapies by monitoring degranulation, mediator release, and cytokine generation.

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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

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Last Updated: Jun 7, 2026

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

Area of Science:

  • Immunology
  • Cell Biology
  • Pharmacology

Background:

  • Mast cells are key players in allergic inflammatory reactions, releasing mediators during anaphylaxis and atopy.
  • Mast cell activation involves immediate degranulation and phospholipid metabolism, followed by delayed cytokine gene expression.

Purpose of the Study:

  • To describe techniques for monitoring mast cell activation in culture systems.
  • To facilitate the understanding of molecular mechanisms in mast cell-mediated diseases.
  • To enable high-throughput screening for novel mast cell inhibitors and therapies.

Main Methods:

  • Monitoring degranulation in mast cell cultures.
  • Assessing phospholipid-derived inflammatory mediator production.
  • Quantifying cytokine generation following mast cell activation.
  • Utilizing biochemical analysis of cell signaling parameters.

Main Results:

  • Established methods for analyzing mast cell degranulation.
  • Quantified phospholipid-derived inflammatory mediator release.
  • Measured cytokine generation in mast cell culture systems.
  • Demonstrated adaptability to rodent and human mast cell systems.

Conclusions:

  • The described techniques are essential for studying mast cell-mediated diseases.
  • These methods support the identification of potential therapeutic targets for allergic diseases.
  • The described approaches are valuable for both basic research and drug discovery.