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Related Experiment Video

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Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy
09:17

Longitudinal Intravital Imaging of Brain Tumor Cell Behavior in Response to an Invasive Surgical Biopsy

Published on: May 3, 2019

Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery.

Yinghua Sun1, Nisa Hatami, Matthew Yee

  • 1University of California, Davis, Department of Biomedical Engineering, Davis, California 95616, USA.

Journal of Biomedical Optics
|November 9, 2010
PubMed
Summary

We introduce an endoscopic fluorescence lifetime imaging microscopy (FLIM) system for real-time glioblastoma multiforme (GBM) diagnosis during surgery. This technology differentiates tumor from healthy tissue, aiding image-guided brain tumor resection.

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Area of Science:

  • Neuroscience
  • Medical Imaging
  • Biophotonics

Background:

  • Glioblastoma multiforme (GBM) is an aggressive brain tumor requiring precise surgical resection.
  • Intraoperative tumor margin delineation remains a challenge in neurosurgery.
  • Advanced imaging techniques are needed to improve surgical outcomes.

Purpose of the Study:

  • To evaluate the feasibility of an endoscopic fluorescence lifetime imaging microscopy (FLIM) system for intraoperative GBM diagnosis.
  • To assess the system's ability to differentiate between GBM and normal brain tissue.
  • To explore FLIM as a tool for image-guided brain tumor surgery.

Main Methods:

  • Development of a clinically compatible endoscopic FLIM prototype with a gated intensifier and fiber-bundle.
  • In vivo experiments on three patients undergoing craniotomy for GBM resection.
  • Analysis of fluorescence intensity and lifetime parameters (τ) at specific wavelengths (460±25 nm) corresponding to NADH/NADPH.

Main Results:

  • The endoscopic FLIM system successfully provided intraoperative access to the surgical field.
  • FLIM parameters effectively delineated GBM from normal brain cortex.
  • GBM tissue showed significantly weaker fluorescence intensity (35% less, p<0.05) and longer fluorescence lifetime (τGBM-Amean=1.59±0.24 ns) compared to normal cortex (τNC-Amean=1.28±0.04 ns, p<0.005).

Conclusions:

  • Endoscopic FLIM shows significant potential for real-time intraoperative diagnosis of GBM.
  • FLIM-derived parameters offer valuable contrast for distinguishing tumor margins.
  • This technology could enhance image-guided surgery for brain tumors, improving resection accuracy.