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A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation
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Model-based quantitative laser Doppler flowmetry in skin.

Ingemar Fredriksson1, Marcus Larsson, Tomas Strömberg

  • 1Linköping University, Department of Biomedical Engineering, University Hospital, 581 85 Linköping, Sweden. ingfr@imt.liu.se

Journal of Biomedical Optics
|November 9, 2010
PubMed
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Quantitative laser Doppler flowmetry (qLDF) offers absolute microcirculatory perfusion measurements, overcoming limitations of conventional LDF. This advanced method accurately quantifies blood flow speed distribution, improving perfusion assessment in tissues.

Area of Science:

  • Biomedical Engineering
  • Optical Diagnostics
  • Physiology

Background:

  • Conventional laser Doppler flowmetry (cLDF) provides relative perfusion estimates.
  • cLDF lacks information on blood flow speed distribution and measurement volume.
  • Limitations of cLDF hinder precise microcirculatory assessment.

Purpose of the Study:

  • To introduce a model-based analysis method for quantitative LDF (qLDF).
  • To enable absolute microcirculatory perfusion determination with blood flow speed information.
  • To overcome the limitations of conventional LDF in perfusion measurement.

Main Methods:

  • Utilized an inverse Monte Carlo technique with an adaptive three-layer skin model.
  • Matched measured and simulated LDF spectra at two source-detector separations.

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  • Determined absolute perfusion within a specified speed region and predefined volume.

    • Main Results:

    • qLDF demonstrated <12% errors with physiological variations and <5% with inhomogeneous models.
    • Residual analysis could dismiss errors from larger blood vessels.
    • In vivo measurements showed higher perfusion increase with qLDF than cLDF due to nonlinearities.

    Conclusions:

    • qLDF provides accurate, absolute microcirculatory perfusion measurements.
    • The method quantifies perfusion based on blood flow speed, particularly for red blood cells >1 mm/s.
    • qLDF offers a significant advancement over cLDF for perfusion assessment.