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Related Concept Videos

Vaccine Production01:23

Vaccine Production

Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...

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An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

Adenovirus vector production and purification.

Ana Carina Silva1, Cristina Peixoto, Tanja Lucas

  • 1IBET/ITQB-UNL, Apartado 12, P-2781-901, Oeiras, Portugal.

Current Gene Therapy
|November 9, 2010
PubMed
Summary
This summary is machine-generated.

Replication deficient adenovirus vectors are key for gene delivery and therapeutic development. This review covers their production, cell lines, and purification processes for optimal use.

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Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
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Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

Published on: January 28, 2016

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • Replication deficient adenovirus vectors are widely employed for transgene delivery in both laboratory and clinical settings.
  • Adenovirus-based therapeutics are advancing through clinical trials, highlighting their therapeutic potential.

Purpose of the Study:

  • To provide a comprehensive overview of adenovirus vector production methodologies.
  • To discuss various types of adenovirus vectors, suitable production cell lines, and current advancements in process development and purification.

Main Methods:

  • Review of existing literature on adenovirus vector production.
  • Analysis of different adenovirus vector systems and their applications.
  • Examination of cell line capabilities for adenovirus vector manufacturing.
  • Evaluation of state-of-the-art purification techniques for adenovirus vectors.

Main Results:

  • Adenovirus vectors are versatile tools for gene therapy applications.
  • Selection of appropriate vector types and production cell lines is crucial for efficient manufacturing.
  • Advanced purification strategies are essential for producing high-quality adenovirus vectors for therapeutic use.

Conclusions:

  • Effective production, purification, and process development are critical for the successful clinical translation of adenovirus-based gene therapies.
  • Continued innovation in adenovirus vector technology will further enhance their therapeutic applications.