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[Study of early pathological changes and axonal injury of optic nerve in experimental allergic encephalomyelitis
1Department of Ophthalmology, The First Affiliated Hospital, China Medical University, Shenyang, China.
Objective:
To observe the early pathological changes and myelin axonal injury of optic nerve during the different stage in EAE mouse, which can provide the theoretical evidence for further therapy.
Methods:
It was an experimental study. EAE was induced with synthetic peptides that represent aa 35 to 55 (MOG35-55) by immunizing C57BL/6 mouse; To observe the pathological change of optic nerve in normal group, EAE group post-immunization 7, 11, 15, 19 d by morphology; The expression of β-APP proteins marked axonal injury and normal myelin proteins CNPase in normal group, EAE group optic nerve after immunization 7, 11, 15, 19 d were detected by immunohistochemistry.
Results:
More and more serious inflammation occurred in the optic nerve of EAE group after immunization 7, 11, 15, 19 d; The expression of CNPase proteins in the optic nerve of EAE group decreased post-immunization 11 d, so are in 15 d, 19 d, and the expression of β-APP proteins in the optic nerve of EAE group increased significantly from 7 d to 19 d post-immunization.
Conclusion:
Inflammation in optic nerve of EAE mouse differ from early stage to peak stage after EAE induction; Axonal damage existing in the optic nerve of EAE mouse is independent on demyelination.
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