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Related Experiment Video

Updated: Jun 7, 2026

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

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Published on: August 11, 2021

Serotonin level and serotonin uptake in human platelets: a variable interrelation under marked physiological

Melita Balija¹, Tatjana Bordukalo-Niksic, Gordana Mokrovic

¹Croatian Institute of Transfusion Medicine, Petrova 3, HR-10 000 Zagreb, Croatia.

Clinica Chimica Acta; International Journal of Clinical Chemistry

|November 9, 2010

Summary

Platelet serotonin level (PSL) and platelet serotonin uptake (PSU) show a weak correlation, influenced by sex, age, and season. Environmental factors like seasons significantly impact this relationship in humans.

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Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

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Area of Science:

Biochemistry
Human Physiology
Pharmacology

Background:

Platelet serotonin level (PSL) is linked to platelet serotonin uptake (PSU).
Existing research on the PSL-PSU interrelation is inconsistent.
A systematic exploration in a large human population is needed.

Purpose of the Study:

To systematically investigate the relationship between platelet serotonin level (PSL) and platelet serotonin uptake (PSU).
To identify factors influencing the correlation between PSL and PSU in a large human cohort.

Main Methods:

Determined PSL and full-kinetics of PSU in 318 blood donors.
Analyzed data considering sex, age, and season of measurement.

Main Results:

Overall correlation between PSL and maximal PSU velocity was significant but low (r=0.269).
Correlation varied by sex, age, and season; absent in females, dependent on age and season in males.
Season was identified as the sole independent predictor of the PSL-PSU relationship.

Conclusions:

Biological (sex, age) and environmental (season) factors significantly influence the intraindividual PSL-PSU relationship.
While serotonin transporter activity is crucial for PSL, other factors likely play a predominant role.
The findings highlight the complexity of serotonin regulation in platelets.