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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibody Structure and Classes01:25

Antibody Structure and Classes

Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.

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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Published on: January 17, 2015

Module based antibody engineering: a novel synthetic REDantibody.

Anatoliy Markiv1, Bernard Anani, Ravi V Durvasula

  • 1Department of Molecular and Applied Biosciences, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London, W1W 6UW, UK.

Journal of Immunological Methods
|November 9, 2010
PubMed
Summary

Researchers developed a stable, red fluorescent recombinant antibody (REDantibody) for immunofluorescence. This novel antibody platform enables rapid screening of antibodies against cell surface markers and allows for interchangeable binding sites and fluorophores.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • Antibodies are crucial for diagnostics and research.
  • Developing fluorescent antibodies often involves complex conjugation methods.
  • There is a need for stable, intrinsically fluorescent antibodies for various applications.

Purpose of the Study:

  • To describe the facile generation of a stable recombinant antibody with intrinsic red fluorescent properties.
  • To validate the REDantibody platform using different antibody specificities.
  • To demonstrate its utility in immunofluorescence analysis.

Main Methods:

  • Designed a REDantibody by integrating red fluorescent protein into antibody variable domains (V(H) and V(L)) based on crystallographic structures.
  • Validated the design using anti-sialyl-Tn, anti-Lewis (Le)(a), and anti-HER2 antibodies.
  • Expressed and purified chimeric antibodies in Escherichia coli, confirming monomeric structure and secretion.

Main Results:

  • Successfully generated stable, monomeric REDantibodies with intrinsic red fluorescence.
  • Demonstrated the utility of REDantibodies in immunofluorescence analysis of Trypanosoma cruzi and HER2.
  • Confirmed the modular nature of the platform for creating diverse fluorescent antibody panels.

Conclusions:

  • The REDantibody platform provides a robust method for generating intrinsically fluorescent antibodies.
  • This technology facilitates rapid screening of antibodies against cell surface markers.
  • The modular design allows for customization with different binding sites and fluorophores for versatile applications.