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Interleukin-2 inhibits growth of fibroblasts derived from human bone marrow

D MacDonald1, J A Adams, D McCarthy

  • 1Department of Haematology, Westminster Hospital, London, UK.

Acta Haematologica
|January 1, 1990
PubMed

Insights

Recombinant interleukin-2 (r-IL2) stops fibroblast colony-forming cells (CFU-F) in bone marrow from growing by blocking their cell cycle entry. This finding suggests r-IL2 impacts human hematopoiesis through its effects on bone marrow CFU-F.

Area of Science:

  • Hematology
  • Cell Biology
  • Immunology

Background:

  • Bone marrow suspension cultures form an adherent layer containing fibroblast colonies.
  • These colonies originate from colony-forming cells (CFU-F).

Purpose of the Study:

  • To investigate the effect of recombinant interleukin-2 (r-IL2) on the growth of CFU-F in human bone marrow cultures.
  • To elucidate the mechanism by which r-IL2 influences CFU-F proliferation.

Main Methods:

  • Human bone marrow suspension cultures were utilized.
  • The impact of r-IL2 on CFU-F growth and cell cycle progression was assessed.
  • Experiments included the use of anti-IL2 receptor antibody and assessments for gamma-interferon and T lymphocytes.

Main Results:

  • Recombinant interleukin-2 (r-IL2) was found to inhibit the growth of CFU-F.
  • This inhibition occurs by preventing CFU-F from entering the S phase of the cell cycle.
  • The inhibitory effect was not mediated by gamma-interferon or T lymphocytes and could be reversed by an anti-IL2 receptor antibody.

Conclusions:

  • r-IL2 directly inhibits the proliferation of bone marrow CFU-F.
  • r-IL2 influences hematopoiesis through its inhibitory action on bone marrow CFU-F.
  • The mechanism involves blocking cell cycle progression into S phase, independent of T cells or gamma-interferon.

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