Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Stem Cell Niche

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

Cells Coordinate Growth and Proliferation

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...

Cells Coordinate Growth and Proliferation

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...

Maintenance of the ES Cell State

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Multipotency and Niche of Bulge Stem Cell

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

mTORC1 is required for differentiation of germline stem cells in the Drosophila melanogaster testis.

PloS one·2024

Same author

The septate junction component bark beetle is required for <i>Drosophila</i> intestinal barrier function and homeostasis.

iScience·2023

Same author

Culture-Associated DNA Methylation Changes Impact on Cellular Function of Human Intestinal Organoids.

Cellular and molecular gastroenterology and hepatology·2022

Same author

Escargot controls somatic stem cell maintenance through the attenuation of the insulin receptor pathway in Drosophila.

Cell reports·2022

Same author

Conditional PD-1/PD-L1 Probody Therapeutics Induce Comparable Antitumor Immunity but Reduced Systemic Toxicity Compared with Traditional Anti-PD-1/PD-L1 Agents.

Cancer immunology research·2021

Same author

Regeneration, Rejuvenation, and Replacement: Turning Back the Clock on Tissue Aging.

Cold Spring Harbor perspectives in biology·2021

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Published on: September 25, 2016

Stem cell dynamics in response to nutrient availability.

Catherine J McLeod¹, Lei Wang, Chihunt Wong

¹Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

Current Biology : CB

|November 9, 2010

Summary

This summary is machine-generated.

Animals adjust metabolism during nutrient scarcity. This study shows adult stem cells in flies reduce proliferation but remain responsive, highlighting their role in maintaining tissue homeostasis under metabolic stress.

More Related Videos

The Use of Chemostats in Microbial Systems Biology

The Use of Chemostats in Microbial Systems Biology

Published on: October 14, 2013

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Related Experiment Videos

Last Updated: Jun 6, 2026

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids

Published on: September 25, 2016

The Use of Chemostats in Microbial Systems Biology

The Use of Chemostats in Microbial Systems Biology

Published on: October 14, 2013

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

Area of Science:

Developmental Biology
Metabolic Regulation
Stem Cell Biology

Background:

Nutrient availability influences animal metabolism and resource allocation.
The response of adult stem cell niches to chronic metabolic changes is not well understood.

Purpose of the Study:

To investigate how germline and intestinal stem cells in flies respond to protein starvation and refeeding.
To identify mechanisms regulating stem cell maintenance during metabolic stress.

Main Methods:

Feeding adult flies a protein-deficient diet (protein starvation).
Assaying germline and intestinal stem cell proliferation and number.
Monitoring stem cell response upon refeeding.
Investigating the role of the insulin/IGF signaling pathway.

Main Results:

Protein starvation decreased stem cell proliferation and number, but a responsive pool remained.
Refeeding led to a significant increase in stem cell number, demonstrating rapid recovery.
Activation of insulin/IGF signaling in stem cells and support cells prevented starvation-induced stem cell loss.

Conclusions:

Adult stem cells can sense systemic metabolic status and adjust their behavior accordingly.
Stem cell maintenance during metabolic stress is regulated by intrinsic and extrinsic factors, including insulin/IGF signaling.
These findings provide a model for how stem cells maintain tissue homeostasis under metabolic challenges.