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Related Experiment Video

Updated: Jun 6, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

EM-ISEL: a useful tool to visualize DNA damage at the ultrastructural level.

Antonio Migheli1

  • 1Centro Regionale Diagnosi ed Osservazione delle Malattie Prioniche DOMP-ASL TO2, Turin, Italy. a.migheli@gmail.com

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2010
PubMed
Summary

This study presents a new method to visualize DNA strand breaks using electron microscopy and colloidal gold. The technique effectively distinguishes various cell death types and DNA damage, even in archival tissues.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Pathology

Background:

  • DNA strand breaks are critical indicators of cellular damage and cell death.
  • Accurate localization of DNA breaks is essential for understanding disease mechanisms.
  • Existing methods may lack the resolution or applicability for diverse conditions.

Purpose of the Study:

  • To develop a novel ultrastructural method for localizing DNA strand breaks.
  • To enable differentiation between apoptotic, necrotic, and other DNA damage types.
  • To assess the technique's utility in routine electron microscopy and archival samples.

Main Methods:

  • Utilized terminal deoxynucleotidyl transferase (TdT) and labeled deoxyuridine triphosphate (dUTP).
  • Employed colloidal gold for visualizing incorporated labeled nucleotides.

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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

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Related Experiment Videos

Last Updated: Jun 6, 2026

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
12:29

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

Published on: March 20, 2018

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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

  • Applied the method to tissues processed for standard electron microscopy.

    • Main Results:

    • Successfully localized DNA strand breaks at the ultrastructural level.
    • Enabled clear distinction between apoptotic, necrotic, and death-unrelated DNA damage.
    • Demonstrated applicability to various pathological conditions and archival tissues.

    Conclusions:

    • The presented technique offers a robust method for ultrastructural DNA break detection.
    • It provides valuable insights into DNA damage and cell death pathways.
    • The method's feasibility for retrospective studies on archival material is confirmed.