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Updated: Jun 6, 2026
Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus
Published on: January 23, 2018
Scanty integration of osteochondral allografts cryopreserved at low temperatures with dimethyl sulfoxide
Francisco Forriol1, Umile Giuseppe Longo, Eduardo Alvarez
1Research Unit FREMAP Hospital, Madrid, Spain.
Purpose:
To compare the integration of osteochondral allografts cryopreserved at different temperatures and different concentrations of dimethyl sulfoxide in an in vivo sheep animal model.
Methods:
Thirty-six adult sheep were randomly allocated to 6 groups of allograft osteochondral transplantation. Six osteochondral cylinders were stored for 6 weeks at -80°C; 6 at -80°C with 10% dimethyl sulfoxide (DMSO); 6 at -80°C with 10% DMSO for 90 min; 6 at -186°C; 6 at -186°C with 10% DMSO; 6 at -186°C for 90 min. After transplantation, all animals were euthanized at 6 months. Harvested specimens underwent gross morphologic and histologic evaluation.
Results:
We found no statistically significant differences when comparing the gross cartilage morphology and histopathologic scores of each group. The Mankin and OARSI scores and the modified Wakitani and OARSI scores showed a good correlation grade. The Mankin and modified Wakitani scores showed a fair correlation grade.
Conclusion:
The cryopreservation protocols adopted in the present study provided scanty integration in an in vivo sheep model of osteochondral allograft transplantation. Therefore, their use in the clinical practice is discouraged.
