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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

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Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
07:46

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Published on: July 28, 2023

Efficient generation of nonhuman primate induced pluripotent stem cells.

Bonan Zhong1, Grant D Trobridge, Xiaobing Zhang

  • 1Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA.

Stem Cells and Development
|November 10, 2010
PubMed
Summary

Researchers generated induced pluripotent stem (iPS) cells from nonhuman primates using a novel gammaretroviral vector system. This breakthrough facilitates the evaluation of iPS cell therapies in large animal models for regenerative medicine applications.

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Gene Therapy

Background:

  • Induced pluripotent stem (iPS) cells hold promise for regenerative medicine and gene therapy.
  • Current iPS cell generation often relies on integrating viral vectors, posing risks like malignant transformation.
  • Non-integrating methods for iPS cell generation have historically been less effective.

Purpose of the Study:

  • To generate and characterize nonhuman primate iPS cells for evaluating transplantation in a clinically relevant large animal model.
  • To develop an efficient and reproducible method for creating nonhuman primate iPS cells.
  • To establish a resource for optimizing iPS cell technology for clinical applications.

Main Methods:

  • Developed stable Phoenix-RD114-based packaging cell lines to produce gammaretroviral vectors expressing OCT4, SOX2, c-MYC, and KLF4 (OSCK).
  • Utilized these vectors in combination with small molecules to reprogram somatic cells from pigtailed macaques (Macaca nemestrina).
  • Characterized the generated nonhuman primate iPS cells for pluripotency and self-renewal capacity.

Main Results:

  • Successfully generated induced pluripotent stem (iPS) cells from nonhuman primates (pigtailed macaques).
  • The generated nonhuman primate iPS cells demonstrated pluripotency and extensive self-renewal capacity.
  • The method proved efficient and reproducible, utilizing defined producer cells for reprogramming factors.

Conclusions:

  • Facile and reproducible generation of nonhuman primate iPS cells is achievable using defined gammaretroviral producer cells.
  • This advancement provides a valuable resource for optimizing and evaluating iPS cell technology.
  • Enables critical studies for the clinical application of iPS cell-based regenerative medicine in large animal models.