Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robot-Assisted Transvesical Enucleation of Benign Prostatic Hyperplasia: Lessons from a Single Surgeon's Learning Curve.

Urology practice·2023
Same author

Long-term results of ProACT primary and repeat implantation for treatment of stress urinary incontinence in men.

World journal of urology·2018
Same author

[Gap between postulated and real outcome quality of radical prostatectomy].

Der Urologe. Ausg. A·2015
Same author

[Vesicovaginal fistula. Incidence, etiology and phenomenology in Germany].

Der Urologe. Ausg. A·2015
Same author

[Minimally invasive vs. open surgical procedures in the treatment of renal cell carcinoma].

Der Urologe. Ausg. A·2015
Same author

[Bone metastasis by renal cell carcinoma. Importance of calcium and calcium-sensing receptor].

Der Urologe. Ausg. A·2014

Related Experiment Video

Updated: Jun 6, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Targeted resequencing of candidate genes using selector probes.

H Johansson1, M Isaksson, E Falk Sörqvist

  • 1Department of Genetics and Pathology, Uppsala University, Rudbeck Laboratory, Uppsala, Sweden.

Nucleic Acids Research
|November 10, 2010
PubMed
Summary

This study introduces an improved Selector technique for targeted genome enrichment, enhancing DNA sequencing efficiency. The method achieves high specificity and coverage for cancer-related genes, enabling copy-number variation detection.

More Related Videos

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Related Experiment Videos

Last Updated: Jun 6, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
09:06

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Published on: October 5, 2018

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

  • Genomics
  • Molecular Biology
  • Cancer Research

Background:

  • Next-generation sequencing (NGS) offers massive throughput but requires efficient methods for targeted genome enrichment.
  • Existing protocols may have limitations in coverage, specificity, or compatibility with diverse sample types.
  • The Selector technique provides a foundation for multiplex amplification of specific DNA regions.

Purpose of the Study:

  • To present a simple, scalable, and updated protocol for multiplex amplification of target DNA regions using the Selector technique.
  • To improve coverage and compatibility with NGS library construction for shotgun sequencing.
  • To demonstrate the efficacy of the enhanced protocol in cancer gene enrichment.

Main Methods:

  • Utilized an updated Selector technique for multiplex amplification of 501 exons from 28 cancer-associated genes.
  • Applied the protocol to DNA from cell lines and both fresh frozen and formalin-fixed paraffin-embedded (FFPE) tumor biopsies.
  • Performed shotgun sequencing on next-generation sequencing (NGS) platforms.

Main Results:

  • Achieved 94% specificity and 98% coverage of targeted genomic regions.
  • Demonstrated high reproducibility between replicates (R(2) = 0.98), enabling copy-number variation detection.
  • The protocol is rapid, completable in under 24 hours, and requires no specialized instrumentation.

Conclusions:

  • The enhanced Selector technique is a robust and efficient method for targeted genome enrichment.
  • The protocol is suitable for various sample types, including challenging FFPE tissues.
  • This technique facilitates deep sequencing of cancer-related genes and supports copy-number variation analysis in clinical and research settings.