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[Gene expression in murine splenocytes induced by soluble beta-glucan]
Toshie Hida1, Hiromi Kawaminami, Ken-ichi Ishibashi
1Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy & Life Sciences.
Nihon Ishinkin Gakkai Zasshi = Japanese Journal of Medical Mycology
|November 10, 2010
Summary
Glucan (SCG) from fungi activates mouse splenocytes, inducing cytokines like GM-CSF, IFN-γ, and TNF-α. Gene expression analysis revealed SCG up-regulates factors including Edn1 and Ptgs2, impacting immune responses.
Area of Science:
- Immunology
- Molecular Biology
- Mycology
Context:
- Scleroglucan (SCG), a fungal cell wall component, is known to activate leukocytes.
- DBA/2 mice leukocytes exhibit high sensitivity to SCG, producing key cytokines.
- Understanding SCG's molecular effects on immune cells is crucial for immune response research.
Purpose:
- To analyze factors induced by SCG in splenocytes from DBA/2 mice.
- To investigate gene expression changes in response to SCG using DNA microarray.
- To determine the kinetics of cytokine production and gene induction by SCG.
Summary:
- SCG stimulation of DBA/2 mouse splenocytes induced cytokines (GM-CSF, IFN-γ, TNF-α) and chemokines.
- DNA microarray analysis revealed SCG up-regulated genes such as Edn1 and Ptgs2.
- Prostaglandin E2 (PGE2) was detected, indicating broader molecular responses beyond cytokines.
Impact:
- Splenocytes show enhanced sensitivity to SCG in early culture periods.
- SCG induces not only cytokines but also a variety of other factors, broadening its known biological activity.
- These findings contribute to understanding fungal-host interactions and immune modulation by β-glucans.
