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Related Concept Videos

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 6, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Extreme-ultraviolet interferometry at 15.5 nm using multilayer optics.

L B Da Silva, T W Barbee, R Cauble

    Applied Optics
    |November 10, 2010
    PubMed
    Summary
    This summary is machine-generated.

    New multilayer mirrors and thin membranes enabled a Mach-Zehnder interferometer for soft x-ray lasers. This device probes high-density plasmas, advancing scientific understanding.

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    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
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    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

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    Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    Area of Science:

    • Optics and Photonics
    • Plasma Physics
    • Materials Science

    Background:

    • Advancements in multilayer mirror technology operating within the 3-30 nm range are crucial for developing novel optical instruments.
    • The fabrication of thin membranes with high uniformity and mechanical strength is essential for creating stable interferometric devices.

    Purpose of the Study:

    • To design and implement a Mach-Zehnder interferometer specifically for operation at a soft x-ray wavelength of 15.5 nm.
    • To demonstrate the utility of this novel interferometer for plasma diagnostics.

    Main Methods:

    • Development of advanced multilayer mirrors for 15.5 nm operation.
    • Construction of robust, uniform thin membranes for interferometer components.
    • Testing the interferometer using a soft x-ray laser source.

    Main Results:

    • Successful design and implementation of a Mach-Zehnder interferometer operating at 15.5 nm.
    • Demonstration of the interferometer's capability to probe high-density plasmas.
    • Validation of the performance of the developed multilayer optics and membranes.

    Conclusions:

    • The developed Mach-Zehnder interferometer represents a significant advancement in soft x-ray optics.
    • This technology provides a new tool for the characterization of high-density plasmas.
    • The combination of advanced multilayer mirrors and thin membranes opens new avenues for soft x-ray applications.