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Related Experiment Video

Updated: Jun 6, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Binary IS typing for Staphylococcus aureus.

Andries E Budding¹, Christina M J E Vandenbroucke-Grauls, Damian C Melles

¹Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands. d.budding@vumc.nl

|November 10, 2010

Summary

Binary interspace (IS) typing offers a rapid and easy method for Staphylococcus aureus identification. This molecular technique shows high discriminatory potential and correlates well with established methods for epidemiological studies.

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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

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Published on: September 5, 2013

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

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Published on: November 4, 2016

Area of Science:

Microbiology
Molecular Biology
Epidemiology

Background:

Staphylococcus aureus requires rapid and reliable typing methods for epidemiological surveillance.
Existing molecular typing techniques can be complex and time-consuming.
A need exists for easily applicable and standardized methods for bacterial identification.

Purpose of the Study:

To introduce and evaluate binary interspace (IS) typing as a novel method for Staphylococcus aureus.
To assess the discriminatory potential and correlation of binary IS typing with established techniques.

Main Methods:

Binary interspace (IS) typing was developed based on length polymorphisms of the 16S-23S rDNA interspace region.
424 Staphylococcus aureus isolates (MRSA and MSSA) from human and animal sources were analyzed.
Binary IS typing results were compared with multi locus sequence typing (MLST) and Amplified Fragment Length Polymorphism (AFLP).

Main Results:

Binary IS typing demonstrated a high discriminatory potential for Staphylococcus aureus isolates.
The method showed a good correlation with multi locus sequence typing (MLST) and Amplified Fragment Length Polymorphism (AFLP).
Binary profiles were generated in a standardized and reproducible manner.

Conclusions:

Binary IS typing is an easily applicable and standardized technique for Staphylococcus aureus.
Its high discriminatory power and correlation with MLST make it suitable for large-scale molecular epidemiological studies.
This method facilitates inter-laboratory comparisons for tracking bacterial strains.