Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Postoperative Delirium Following Continuous Fascia Iliaca Compartment Block in Elderly Patients with Hip Fracture: A Case Series and Literature Review.

Local and regional anesthesia·2026
Same author

Enabling and Enhancing Massive Multiple Input-Multiple Output Systems with Two-Dimensional Orthogonal Pattern Division Multiple Access.

Sensors (Basel, Switzerland)·2026
Same author

Identification and transcriptome analysis of a major locus for eye depth in tetraploid potato Jinshu 16.

BMC plant biology·2026
Same author

Preparation and Application of Cellulose-Based Thermosensitive Polymer in Water-Based Drilling Fluid.

Polymers·2026
Same author

Biomechanical comparison of frame configuration screw fixation and conventional plate fixation for Sanders type iii fractures: a finite element analysis.

BMC surgery·2026
Same author

Population-scale repeat expansions elucidate disease risk and brain atrophy.

Nature·2026

Related Experiment Video

Updated: Jun 6, 2026

Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR
11:22

Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR

Published on: May 4, 2010

Tissue-specific transcriptomics of the exotic invasive insect pest emerald ash borer (Agrilus planipennis).

Omprakash Mittapalli1, Xiaodong Bai, Praveen Mamidala

  • 1Department of Entomology, Ohio Agricultural and Research Development Center, The Ohio State University, Wooster, Ohio, United States of America. mittapalli.1@osu.edu

Plos One
|November 10, 2010
PubMed
Summary

This study reveals tissue-specific gene expression in the invasive emerald ash borer (Agrilus planipennis), identifying key genes for digestion and detoxification. These findings provide a molecular basis for understanding and managing this destructive forest pest.

More Related Videos

Building Double-decker Traps for Early Detection of Emerald Ash Borer
06:07

Building Double-decker Traps for Early Detection of Emerald Ash Borer

Published on: October 4, 2017

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
12:10

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Published on: November 30, 2014

Related Experiment Videos

Last Updated: Jun 6, 2026

Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR
11:22

Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR

Published on: May 4, 2010

Building Double-decker Traps for Early Detection of Emerald Ash Borer
06:07

Building Double-decker Traps for Early Detection of Emerald Ash Borer

Published on: October 4, 2017

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
12:10

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Published on: November 30, 2014

Area of Science:

  • Entomology
  • Molecular Biology
  • Genomics

Background:

  • The emerald ash borer (Agrilus planipennis) is a destructive invasive insect pest impacting ash trees.
  • Limited molecular-level knowledge exists for A. planipennis despite its significant ecological and economic impact.
  • Insect midgut and fat body are crucial for digestion, detoxification, and immunity.

Purpose of the Study:

  • To characterize tissue-specific gene expression in the emerald ash borer (A. planipennis).
  • To identify molecular targets for pest management strategies.
  • To lay the groundwork for future functional genomic studies in A. planipennis.

Main Methods:

  • Utilized Roche-454 pyrosequencing to generate expressed sequence tags (ESTs) from A. planipennis larval midgut and fat body.
  • Assembled and analyzed ESTs to identify gene functions, domains, single nucleotide polymorphisms (SNPs), and microsatellite loci.
  • Examined tissue-specific expression patterns of cytochrome P450 genes.

Main Results:

  • Generated over 25,000 high-quality ESTs for the midgut and over 37,000 for the fat body.
  • Identified significant numbers of transcripts related to chitin-binding peritrophins and trypsin in the midgut, and cytochrome P450s and protein kinases in the fat body.
  • Discovered putative microbial transcripts in the midgut and predicted numerous SNPs and microsatellite loci across both tissues, with distinct cytochrome P450 expression.

Conclusions:

  • This study provides the first comprehensive insight into tissue-specific gene expression in the invasive emerald ash borer.
  • The identified genes and genetic markers are crucial for future functional and gene expression studies.
  • Findings will advance our understanding of A. planipennis biology and support the development of effective pest management strategies.