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Published on: August 2, 2018
Phosphoproteome profile of human lung cancer cell line A549
Guangchuang Yu1, Chuan-Le Xiao, Chun-Hua Lu
1Institute of Life and Health Engineering, Jinan University, Guangzhou 510632, China.
Abstract:
As an in vitro model for type II human lung cancer, A549 cells resist cytotoxicity via phosphorylation of proteins as demonstrated by many studies. However, to date, no large-scale phosphoproteome investigation has been conducted on A549. Here, we performed a systematical analysis of the phosphoproteome of A549 by using mass spectrometry (MS)-based strategies. This investigation led to the identification of 337 phosphorylation sites on 181 phosphoproteins. Among them, 67 phosphoproteins and 230 phosphorylation sites identified appeared to be novel with no previous characterization in lung cancer. Based on their known functions as reported in the literature, these phosphoproteins were functionally organized into highly interconnected networks. Western blotting and immunohistochemistry analyses were performed to validate the expression of a bottleneck phosphoprotein YAP1 in cancer cell lines and tissues. This dataset provides a valuable resource for further studies on phosphorylation and lung carcinogenesis.
Insights
This study presents the first large-scale phosphoproteome analysis of A549 lung cancer cells, identifying novel phosphorylation sites and proteins crucial for lung carcinogenesis research.
Area of Science:
- Proteomics
- Cancer Biology
- Molecular Oncology
Background:
- A549 cells are an in vitro model for type II human lung cancer.
- These cells exhibit cytotoxicity resistance through protein phosphorylation.
- No comprehensive phosphoproteome analysis had been performed on A549 cells previously.
Purpose of the Study:
- To conduct a systematic, large-scale phosphoproteome analysis of A549 lung cancer cells.
- To identify novel phosphoproteins and phosphorylation sites involved in lung cancer.
- To provide a foundational dataset for understanding lung carcinogenesis.
Main Methods:
- Mass spectrometry (MS)-based strategies were employed for phosphoproteome profiling.
- Bioinformatic approaches were used to organize identified phosphoproteins into networks.
- Western blotting and immunohistochemistry validated key phosphoprotein expression.
Main Results:
- Identified 337 phosphorylation sites on 181 phosphoproteins in A549 cells.
- Discovered 67 novel phosphoproteins and 230 novel phosphorylation sites in the context of lung cancer.
- Validated the expression of YAP1, a critical phosphoprotein, in cancer tissues and cell lines.
Conclusions:
- This phosphoproteomic dataset offers significant insights into lung cancer biology.
- The identified phosphoproteins and networks represent potential targets for therapeutic intervention.
- This study establishes a valuable resource for future research on lung cancer phosphorylation and development.
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