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Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
Published on: July 20, 2011
Gene expression profile in rat dorsal root ganglion following sciatic nerve injury and systemic neurotrophin-3
Lu-Ting Kuo1, Shao-Yu Tsai, Michael J Groves
1Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Yun-lin branch, No.579, Sec. 2, Yun-lin Rd., Dou-liou City, Yun-lin County, 640, Taiwan. kuoluting@yahoo.com.tw
Abstract:
Following sciatic nerve transection in adult rats, a proportion of injured dorsal root ganglion (DRG) neurons die, through apoptosis, over the following 6 months. Previous studies showed that axotomy and neurotrophin-3 administration may have effects on expression of neurotrophins and their receptors in DRG. In the current study, the fourth and fifth lumbar DRGs of rats were examined 2 weeks after right sciatic nerve transection and ligation. The effects of axotomy and systemic NT-3 treatment on neuronal genes were investigated by microarray. The results demonstrated that bone morphogenetic protein (BMP) and Janus protein tyrosine kinase signaling pathways are induced in axotomized DRG, and PI-3 kinase and BMP pathways and genes controlling various cellular functions were induced after axotomy and NT-3 administration.
