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Updated: Jun 6, 2026

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Published on: April 1, 2016
The euryhaline yeast Debaryomyces hansenii has two catalase genes encoding enzymes with differential activity profile
Claudia Segal-Kischinevzky1, Beatriz Rodarte-Murguía, Victor Valdés-López
1Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, UNAM, Mexico, Mexico. claudia.segal@gmail.com
Abstract:
Debaryomyces hansenii is a spoilage yeast able to grow in a variety of ecological niches, from seawater to dairy products. Results presented in this article show that (i) D. hansenii has an inherent resistance to H2O2 which could be attributed to the fact that this yeast has a basal catalase activity which is several-fold higher than that observed in Saccharomyces cerevisiae under the same culture conditions, (ii) D. hansenii has two genes (DhCTA1 and DhCTT1) encoding two catalase isozymes with a differential enzymatic activity profile which is not strictly correlated with a differential expression profile of the encoding genes.
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