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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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Related Experiment Video

Updated: Jun 6, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

B-cell display-based one-step method to generate chimeric human IgG monoclonal antibodies.

Waka Lin¹, Kohei Kurosawa, Akiho Murayama

¹Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan.

Nucleic Acids Research

|November 11, 2010

Summary

Researchers developed a novel ADLib system application for rapid, one-step isolation of antigen-specific chimeric human monoclonal antibodies (mAbs). This advance streamlines antibody production for therapeutic applications.

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Last Updated: Jun 6, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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Published on: February 6, 2018

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

Area of Science:

Immunology
Molecular Biology
Biotechnology

Background:

Monoclonal antibodies (mAbs) are crucial for research and therapy.
In vitro isolation of mAbs has advanced with antibody fragment display technologies.
The ADLib (Autonomously Diversifying Library) system enables rapid isolation of antigen-specific mAbs from chicken IgM libraries.

Purpose of the Study:

To develop a novel application of the ADLib system for producing chimeric human mAbs.
To generate DT40 cell lines coexpressing chimeric human IgG and chicken IgM.
To demonstrate one-step selection of antigen-specific human chimeric IgG using the modified ADLib system.

Main Methods:

Designed gene knock-in constructs for DT40 strains.
Utilized B-cell-specific RNA alternative splicing for coexpression of human IgG and chicken IgM.
Applied the ADLib system for selection of antigen-specific human chimeric IgG.
Modulated RNA processing by overexpressing CstF-64 to increase chimeric IgG production.

Main Results:

Successfully generated DT40 strains coexpressing chimeric human IgG and chicken IgM.
Demonstrated one-step selection of antigen-specific human chimeric IgG using the ADLib system.
Showed that overexpressing CstF-64 selectively increases chimeric IgG production.

Conclusions:

The modified ADLib system provides an efficient method for producing chimeric human mAbs.
This approach facilitates the design of mAbs for diverse applications, including antibody therapy.
The system offers a streamlined process for generating therapeutic antibodies.