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Related Experiment Video

Updated: Jun 6, 2026

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

New immunoassay platform utilizing yeast surface display and direct cell counting.

Yongchao Guo, Danhui Cheng, Tsz Yan Lee

    Analytical Chemistry
    |November 12, 2010
    PubMed
    Summary

    We developed a novel yeast cell surface display immunoassay for ultrasensitive antibody detection. This method achieves a very low limit of detection and enables multiplexed detection for diagnostics.

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    Area of Science:

    • Biotechnology
    • Immunotechnology
    • Cell Biology

    Background:

    • Conventional immunoassays like ELISA rely on detecting labels, which can limit sensitivity.
    • There is a need for more sensitive and multiplexed immunoassay platforms for diagnostic applications.

    Discussion:

    • A new yeast cell surface display based cell counting immunoassay (YSD-CCI) was developed.
    • This method quantifies antibody analytes by directly counting modified yeast cells, bypassing traditional label detection.
    • Epitope tagging using c-myc and hemagglutinin (HA) demonstrated the platform's efficacy.

    Key Insights:

    • The YSD-CCI achieved a 0.2 ng/mL detection limit for anti-c-myc, significantly outperforming conventional yeast ELISA.
    • The platform successfully demonstrated 2-Plex antibody detection by simultaneously identifying anti-c-myc and anti-HA.

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    Last Updated: Jun 6, 2026

    Protein Engineering by Yeast Surface Display
    05:49

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    Published on: November 29, 2024

    Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
    10:54

    Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

    Published on: May 30, 2025

    A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
    02:54

    A Rapid Technique for the Visualization of Live Immobilized Yeast Cells

    Published on: November 9, 2006

  • Engineered yeast cells expressing EGFP and mCherry facilitated multiplexed detection.

    • Outlook:

    • This proof-of-concept study introduces a promising ultrasensitive immunoassay technique.
    • The YSD-CCI platform has potential for developing advanced diagnostic tools.
    • Further development could lead to broader applications in biomedical research and clinical diagnostics.