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Related Experiment Video

Updated: Jun 6, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Transgenic mice: beyond the knockout.

R Lance Miller¹

¹Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA. lance.miller@nih.gov

American Journal of Physiology. Renal Physiology

|November 12, 2010

Summary

This summary is machine-generated.

Transgenic mice offer diverse applications beyond simple knockouts. Novel transgenic models enable in-vivo studies of molecular pathways, cell interactions, and real-time cell cycle analysis, advancing biomedical research.

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Last Updated: Jun 6, 2026

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Area of Science:

Biomedical Research
Genetics
Molecular Biology

Background:

Transgenic mice are widely used in biomedical research, particularly those expressing Cre recombinase (Cre) for conditional knockouts.
Familiarity with diverse transgenic mouse applications beyond knockouts is limited among researchers.

Purpose of the Study:

To highlight the underutilized applications of various transgenic mouse models.
To demonstrate how novel transgenic mice facilitate in-vivo studies of molecular and biochemical events.

Main Methods:

Utilizing transgenic mice engineered for specific research purposes.
Deriving primary cultures and cell suspensions from transgenic mice.
Employing multiple fluorescent proteins within the same mouse for cell-cell interaction studies.
Performing real-time cell cycle analysis and deep tissue imaging in whole animals.

Main Results:

Transgenic mice enable the study of molecular pathways in primary cultures and cell suspensions.
Applications include observing cell-cell interactions using multiple fluorescent proteins.
Real-time cell cycle analysis and deep tissue imaging are feasible in whole animals.
Cell lineage tracking during development and disease, and isolation of pure cell types from organs are also demonstrated.

Conclusions:

Transgenic mice possess a broader range of applications than commonly recognized.
These models provide powerful tools for studying complex biological processes in a whole-animal context.
Novel transgenic approaches overcome limitations previously confined to cell culture studies.