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Structural studies of Hantaan virus.

Anthony J Battisti¹, Yong-Kyu Chu, Paul R Chipman

¹Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907-2054, USA.

Journal of Virology

|November 12, 2010

Summary

Hantaan virus, a cause of hemorrhagic fever with renal syndrome, has a unique internal structure. Cryo-electron microscopy reveals ordered glycoprotein spikes and internal ribonucleoprotein complexes, differing from other bunyaviruses.

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Area of Science:

Virology
Structural Biology
Microscopy

Background:

Hantaan virus is a prototypic member of the Hantavirus genus (family Bunyaviridae), causing hemorrhagic fever with renal syndrome.
Bunyaviridae are negative-sense RNA viruses with segmented genomes, enveloped virions, and glycoprotein spikes (Gn and Gc).

Purpose of the Study:

To determine the structural organization of Hantaan virus virions using advanced microscopy techniques.
To elucidate the arrangement of surface glycoproteins and internal components within the virion.

Main Methods:

Cryo-electron tomography (cryo-ET) and single-particle cryo-electron microscopy (cryo-EM).
High-resolution structural determination of the Gn-Gc spike complex.

Main Results:

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The tetrameric Gn-Gc spike complex structure was resolved to 2.5 nm, showing ordered lattices on the virion surface.
Cytoplasmic extensions of Gn-Gc spikes also formed a lattice on the inner viral membrane.
Internal rod-shaped ribonucleoprotein complexes were arranged in parallel pairs and triplets, differing from icosahedral structures in some bunyaviruses.

Conclusions:

Hantaan virus exhibits a distinct structural organization compared to some other bunyaviruses.
The findings suggest a common structural framework among hantaviruses, as indicated by comparison with Tula hantavirus.
This structural information is crucial for understanding hantavirus biology and pathogenesis.