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Aplysia Ganglia Preparation for Electrophysiological and Molecular Analyses of Single Neurons
Published on: January 13, 2014
A first partial Aplysia californica proteome.
Yanwei Sun1, Francisco J Monje, Daniela D Pollak
1Department of Pediatrics, Medical University of Vienna, Währinger Gürtel 18-20, 1090, Vienna, Austria.
Amino Acids
|November 12, 2010
Summary
This study presents the first systematic protein profiling of Aplysia californica ganglia, identifying 118 individual proteins and revealing significant differences in protein expression across various ganglia, crucial for neurobiology research.
Area of Science:
- Neuroscience
- Proteomics
- Molecular Biology
Background:
- Aplysia californica (AC) proteins lack systematic study.
- Understanding AC protein expression is vital for neurobiological research.
Purpose of the Study:
- To perform comprehensive protein profiling of Aplysia californica ganglia.
- To identify proteins and analyze differential expression patterns.
Main Methods:
- Protein extraction from AC ganglia.
- Two-dimensional electrophoresis (2DE) and nano-liquid chromatography-electrospray ionization-tandem mass spectrometry (nano-LC-ESI-MS/MS).
- Identification of proteins using a public Aplysia EST database.
Main Results:
- Identified 118 individual proteins from 408 spots, with 14-80% sequence coverage.
- Detected significant differences in protein levels related to signaling, metabolism, cytoskeleton, redox, and other functions across ganglia.
- Generated a protein map complementing existing transcriptional data.
Conclusions:
- The protein map provides a foundation for studying post-translational modifications and splice variants in AC.
- Differential protein expression between ganglia offers insights for future AC neurobiology studies.
- This work establishes a baseline for AC proteomic research.
