[Biochemical study of normal and abnormal thyroid cells in tissue culture (author's transl)]

Thyrotropin induces the formation of a follicular structure of thyroid cells in tissue culture. It produces an increase in cyclic AMP content of thyrotropin treated cells. Thyrotropin induces on increase in iodine incorporation with the appearance of iodinated proteins which are mostly 19 S. thyroglobulin. In goiters, the level of cyclic AMP and the stimulation with thyrotropin differs from normal conditions indicating differences in cell metabolism and differences in the properties of the receptor. Thyrotropin also gives an increase in uridine incorporation in RNA and on increase not only in poly A but also in poly A-RNA associated with heavy polysomes. The level of poly A is higher in toxic adenoma and lower in microfollicular adenoma as compared to colloido nodular goiter which is in good agreement with our knowledge of thyroid cell metabolism.