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Updated: Jun 5, 2026
08:41
Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
Published on: June 7, 2017
L-selectin is dispensable for T regulatory cell function postallogeneic bone marrow transplantation
M J Carlson1, L M Fulton, J M Coghill
1Department of Medicine and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.
Summary
CD62L is not essential for regulatory T cells (Tregs) to prevent graft-versus-host disease (GvHD). Studies show CD62L-deficient Tregs effectively suppress GvHD, challenging previous assumptions about this molecule's role.
Area of Science:
- Immunology
- Transplantation Immunology
Background:
- Adoptive transfer of CD4(+) /CD25(+) regulatory T cells (Tregs) can inhibit graft-versus-host disease (GvHD).
- The adhesion molecule L-selectin (CD62L) has been implicated in Treg function and GvHD prevention.
Purpose of the Study:
- To investigate the role of CD62L in the capacity of Tregs to inhibit acute GvHD.
- To compare the efficacy of wild-type (WT), CD62L(-/-), and CD62L(Lo) Tregs in preventing GvHD.
Main Methods:
- Adoptive transfer of naive WT, CD62L(-/-), and ex vivo expanded CD62L(Lo) Tregs into murine models of GvHD.
- Assessment of GvHD inhibition, organ pathology, cytokine production, and Treg migration.
Main Results:
- CD62L(-/-) Tregs were potent suppressors of GvHD, while CD62L(Lo) Tregs were ineffective.
- WT and CD62L(-/-) Tregs reduced liver pathology and systemic inflammation, but CD62L(-/-) Tregs were less effective in reducing lung pathology.
- CD62L(-/-) Tregs showed equivalent organ accumulation but reduced migration to peripheral lymph nodes compared to WT Tregs.
Conclusions:
- CD62L is dispensable for Treg-mediated protection from GvHD.
- Tregs lacking CD62L can still effectively suppress GvHD, indicating alternative mechanisms of action.
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