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Updated: Jun 6, 2026

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
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Published on: December 29, 2017

SETting the clock for histone H4 monomethylation

Jennifer Lee1, Pengbo Zhou

  • 1Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA.

Molecular Cell
|November 13, 2010
PubMed

Insights

The histone methyltransferase PR-Set7/Set8, crucial for gene regulation, is controlled by the CRL4(Cdt2) ubiquitin ligase. This regulation is dependent on PCNA, a key player in DNA replication.

Area of Science:

  • Epigenetics and Gene Regulation
  • Ubiquitin-Mediated Protein Degradation
  • Chromatin Biology

Background:

  • Histone modifications, such as methylation, play a critical role in regulating gene expression.
  • PR-Set7/Set8 is a key enzyme responsible for H4K20 methylation, impacting chromatin structure.
  • The CRL4(Cdt2) ubiquitin ligase complex is involved in targeting proteins for degradation during the cell cycle.

Discussion:

  • This study elucidates the posttranslational regulation of PR-Set7/Set8, a critical histone methyltransferase.
  • The findings reveal that the PCNA-dependent CRL4(Cdt2) ubiquitin ligase targets PR-Set7/Set8 for regulation.
  • This mechanism links DNA replication (via PCNA) to epigenetic control (via H4K20 methylation).

Key Insights:

  • PR-Set7/Set8 is subject to posttranslational modification by the CRL4(Cdt2) ubiquitin ligase.
  • Proliferating cell nuclear antigen (PCNA) dependence is essential for CRL4(Cdt2) mediated regulation of PR-Set7/Set8.
  • This regulatory pathway ensures proper H4K20 methylation levels during DNA replication.

Outlook:

  • Further investigation into the precise mechanisms of PR-Set7/Set8 ubiquitination and degradation.
  • Exploring the functional consequences of PCNA-dependent CRL4(Cdt2) regulation of PR-Set7/Set8 in various cellular contexts.
  • Potential therapeutic implications targeting this regulatory axis in diseases associated with aberrant gene expression.

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