Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Fixed-bed adsorption characteristics of chlorinated hydrocarbon vapors onto hydrophobic hypercrosslinked polymeric resin].

Huan jing ke xue= Huanjing kexue·2011
Same author

Cofactor binding evokes latent differences in DNA binding specificity between Hox proteins.

Cell·2011
Same author

Decreased Th17 and antigen-specific humoral responses in CX₃ CR1-deficient mice in the collagen-induced arthritis model.

Arthritis and rheumatism·2011
Same author

Integrated sample cleanup and microchip capillary array electrophoresis for high-performance forensic STR profiling.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

[Effects of seed dressing with imidacloprid on the seedlings growth and protective enzyme activities of high-yielding summer maize].

Ying yong sheng tai xue bao = The journal of applied ecology·2011
Same author

Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria.

Lab on a chip·2011

Related Experiment Video

Updated: Jun 6, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Integrated sample cleanup and capillary array electrophoresis microchip for forensic short tandem repeat analysis.

Peng Liu1, James R Scherer, Susan A Greenspoon

  • 1UCSF/UC Berkeley Joint Graduate Group in Bioengineering, University of California, Berkeley, CA 94720, USA.

Forensic Science International. Genetics
|November 13, 2010
PubMed
Summary

A novel capillary array electrophoresis microsystem enhances forensic DNA analysis with inline capture injection, achieving high sensitivity for short tandem repeat profiling. This method significantly improves low-copy-number DNA detection in forensic investigations.

More Related Videos

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

Related Experiment Videos

Last Updated: Jun 6, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
11:49

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
08:22

A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

Published on: December 1, 2017

Area of Science:

  • Forensic Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Short tandem repeat (STR) analysis is crucial for forensic identification.
  • Conventional capillary electrophoresis (CE) methods can face limitations in sensitivity and throughput for forensic samples.
  • Low-copy-number (LCN) DNA analysis presents significant challenges due to limited sample availability.

Purpose of the Study:

  • To develop and validate a high-sensitivity capillary array electrophoresis (CAE) microsystem for forensic STR analysis.
  • To integrate an efficient inline capture injection process with CAE for improved DNA analysis.
  • To assess the microsystem's performance for low-copy-number (LCN) and degraded DNA samples.

Main Methods:

  • Development of a twelve-lane CAE microsystem with a radial microfabricated format.
  • Implementation of an inline capture injection process using a photopolymerized gel plug for biotin-labeled STR amplicons and streptavidin-biotin binding.
  • Automated washing and thermal release of captured DNA for CE separation.
  • Comparison of capture inline injection with conventional cross injection.

Main Results:

  • The microsystem achieved high-throughput analysis of 12 STR samples in 30 minutes without manual intervention.
  • Capture inline injection demonstrated at least a 10-fold improvement in sensitivity compared to conventional cross injection.
  • The limit-of-detection was determined to be 35 haploid copies (17-18 diploid copies) of input DNA.
  • Successful analysis of LCN DNA from degraded samples (touch evidence from water-submerged bullet cartridges) was achieved, yielding significant portions of DNA profiles.

Conclusions:

  • The developed capture-CAE microsystem offers a robust and highly sensitive platform for forensic DNA analysis.
  • The inline capture injection method significantly enhances sensitivity, particularly for LCN and degraded DNA samples.
  • This technology advances forensic capabilities for challenging DNA evidence analysis.