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Updated: Jun 6, 2026
A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Quantification of phytochelatins in Chlamydomonas reinhardtii using ferrocene-based derivatization
Anja Bräutigam1, Susanne Bomke, Thorben Pfeifer
1Martin-Luther-Universität Halle-Wittenberg, Institut für Biochemie und Biotechnologie, Ökologische und Pflanzen-Biochemie, Kurt-Mothes-Straße 3, Halle (Saale), Germany.
Abstract:
A method for the identification and quantification of canonic and isoforms of phytochelatins (PCs) from Chlamydomonas reinhardtii was developed. After disulfide reduction with tris(2-carboxyethyl)phosphine (TCEP) PCs were derivatized with ferrocenecarboxylic acid (2-maleimidoyl)ethylamide (FMEA) in order to avoid oxidation of the free thiol functions during analysis. Liquid chromatography (LC) coupled to electrospray mass spectrometry (ESI-MS) and inductively coupled plasma-mass spectrometry (ICP-MS) was used for rapid and quantitative analysis of the precolumn derivatized PCs. PC(2-4), CysGSH, CysPC(2-4), CysPC(2)desGly, CysPC(2)Glu and CysPC(2)Ala were determined in the algal samples depending on the exposure of the cells to cadmium ions.
