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Distribution of human rotavirus G and P genotypes in a hospital setting from Northern India

Anita Chakravarti1, Mayank Singh Chauhan, Anju Sharma

  • 1Department of Microbiology, Maulana Azad Medical College and Associated Lok Nayak Hospital, New Delhi, India. anitachakravarti@gmail.com

The Southeast Asian Journal of Tropical Medicine and Public Health
|November 16, 2010
PubMed

Insights

Rotavirus G1 and P[8] genotypes were most common in hospitalized children with diarrhea. This finding is crucial for rotavirus vaccine development and surveillance efforts.

Area of Science:

  • Virology
  • Pediatrics
  • Epidemiology

Background:

  • Rotavirus gastroenteritis causes severe dehydrating diarrhea in children globally.
  • G and P genotyping of rotavirus is vital for surveillance and vaccine development.

Purpose of the Study:

  • To determine the predominant G and P genotypes of rotavirus in hospitalized children.
  • To understand the common G-P combinations and mixed infections.

Main Methods:

  • ELISA was used to detect rotavirus in 318 of 862 stool samples.
  • Semi-nested multiplex RT-PCR was employed for G and P genotyping of 100 positive samples.

Main Results:

  • G1 (60%) was the predominant G genotype, followed by G2 (16%), G9 (8%), and G3 (3%).
  • P[8] (40%) was the most common P genotype, followed by P[4] (26%) and P[6] (17%).
  • The most frequent G-P combination was G1P[8] (26%), with 28% of strains showing mixed infections.

Conclusions:

  • G1 and P[8] are the leading rotavirus genotypes in this pediatric population.
  • The identification of G12 genotype warrants further investigation and surveillance.

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