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Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

Patterning of quantum dot bioconjugates via particle lithography.

Zachary R Taylor¹, Ernest S Sanchez, Joel C Keay

¹University of Oklahoma Bioengineering Center, University of Oklahoma, 100 East Boyd, Norman, Oklahoma 73019, United States.

Langmuir : the ACS Journal of Surfaces and Colloids

|November 16, 2010

Summary

We developed a simple method to create ordered arrays of quantum dot bioconjugates (QDBCs) on glass. This technique enables sensitive QDBC-based binding assays for biological molecule detection.

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Area of Science:

Biotechnology
Materials Science
Nanotechnology

Background:

Quantum dot bioconjugates (QDBCs) offer unique optical properties for biological applications.
Ordered arrays of QDBCs are desirable for high-sensitivity assays but challenging to fabricate.

Purpose of the Study:

To present a straightforward technique for creating hexagonally ordered QDBC arrays.
To demonstrate the versatility and applicability of these QDBC arrays in biological assays.

Main Methods:

Particle lithography was employed to create periodic holes in a silane layer on glass coverslips.
QDBCs conjugated to biotin, streptavidin, and anti-mouse IgG were adsorbed into these patterned holes.
The site density was controlled by adjusting QDBC solution concentration.

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Compact Quantum Dots for Single-molecule Imaging

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Published on: October 9, 2012

Production and Targeting of Monovalent Quantum Dots

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Main Results:

Hexagonally ordered QDBC arrays with 500-600 nm diameter adsorption regions were successfully fabricated.
QDBC adsorption diameter was independent of the specific bioconjugate used.
A QDBC-based binding assay demonstrated a wide working concentration range and sub-picomolar detection limit.

Conclusions:

The developed technique provides a simple and versatile method for fabricating ordered QDBC arrays.
These QDBC arrays are suitable for developing highly sensitive biological binding assays.
The method allows for tunable QDBC site density for assay optimization.