Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Inhibitors Of Virion Release

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...

Inhibitors of Virion Maturation and Assembly

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...

Cross-reactivity

Cross-reactivity

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside Inhibitors

Antiviral Nucleoside InhibitorsAntiviral nucleoside inhibitors are structural analogs of natural nucleosides that interfere with viral DNA or RNA synthesis. These compounds selectively target viral polymerases due to their resemblance to host nucleosides, thereby disrupting viral genome replication.Mechanism of Acyclovir ActionAcyclovir is a guanosine analog with a three-carbon acyclic side chain. It selectively targets herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2),...

Inhibitors of Viral Protein Synthesis

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lassa and Mopeia viruses produce different RIG-I-activating RNA in the absence of a functional viral exoribonuclease domain.

Journal of virology·2026

Same author

Searching for immune correlates in Lassa vaccine development - workshop report.

NPJ vaccines·2026

Same author

[Immunosuppression and severe Lassa fever].

Medecine sciences : M/S·2026

Same author

Despite good intentions, the regulation on in vitro diagnostic medical devices (IVDR) in Europe could impact negatively on preparedness and response for the next pandemic.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2026

Same author

A Mammarenavirus Close to Lassa Virus in a Patient with Meningoencephalitis.

The New England journal of medicine·2025

Same author

Expansion of myeloid suppressor cells and suppression of Lassa virus-specific T cells during fatal Lassa fever.

PLoS pathogens·2025

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

Towards broad protection against Ebolaviruses.

Sylvain Baize¹

¹Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, IFR128 Biosciences Gerland, Lyon Sud, 21 av. Tony Garnier, 69365 Lyon cedex 7, France. sylvain.baize@inserm.fr

Future Microbiology

|November 16, 2010

Summary

Researchers developed a novel vaccine platform using DNA vectors and adenoviruses to protect monkeys against a new Ebola virus species. This proof-of-concept study shows potential for broad-spectrum filovirus vaccines.

More Related Videos

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Related Experiment Videos

Last Updated: Jun 6, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Published on: September 27, 2014

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Area of Science:

Virology
Vaccinology
Infectious Diseases

Background:

Ebola and Marburg viruses (filoviruses) cause severe hemorrhagic fevers with no approved vaccines or treatments.
Recent emergence of a new Ebolavirus species underscores the urgent need for broad-spectrum filovirus countermeasures.
Sub-Saharan Africa faces recurrent, significant public health challenges from filovirus outbreaks in humans and primates.

Discussion:

Hensley et al. utilized a robust vaccine platform employing DNA vectors and replication-defective adenoviruses engineered to express Ebolavirus glycoproteins.
This platform was tested for its efficacy in protecting cynomolgus monkeys against a heterologous challenge with a novel Ebolavirus species.
The study demonstrates the potential of this approach to confer cross-protective immunity against diverse filoviruses.

Key Insights:

A novel vaccine strategy using adenoviral vectors successfully protected nonhuman primates against a new Ebolavirus species.
The experimental vaccine demonstrated efficacy against a heterologous filovirus challenge, indicating broad-spectrum potential.
This research provides a critical proof-of-concept for developing pan-filovirus vaccines.

Outlook:

Further research and development are necessary to translate this experimental vaccine approach for human clinical application.
The study highlights the potential for broad protection against evolving filoviruses, addressing a critical public health need.
Continued investigation into vector-based vaccines is crucial for combating emerging infectious diseases like Ebola.