Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vacuolar and ER-Ca2+-ATPases regulate calcium dynamics during pollen tube growth in Arabidopsis thaliana.

Plant physiology·2026
Same author

New Perspectives Provided by Merging Computed Tomographic Scanning and Electroanatomical Mapping of Koch's Pyramid.

Journal of cardiovascular development and disease·2026
Same author

Phantom for standardization in functional near-infrared spectroscopy, part 2: optical properties and Monte Carlo simulations.

Neurophotonics·2026
Same author

Amplitude- and Phase-Programmable Dual-Color Photonic Chip for High-Contrast Structured Illumination Microscopy.

ACS photonics·2026
Same author

MAcro Plant Projection Imaging (MAPPI): An open, scalable platform for whole-plant fluorescence real-time imaging.

Science advances·2026
Same author

In-vivo optical properties spectra across five body locations on ten subjects using time-domain diffuse optics.

Scientific data·2026
Same journal

EXPRESS: Deterministic Compressed Sensing in Time-Domain Spectroscopy.

Applied spectroscopy·2026
Same journal

EXPRESS: Multi-Parameter Wavelength Characterization of Array Spectrometers Under Near-Limit Sampling Conditions.

Applied spectroscopy·2026
Same journal

EXPRESS: A Validated Reference Database for Twentieth-Century Cd-Based Pigments: Integrated Structural and Compositional Characterization.

Applied spectroscopy·2026
Same journal

EXPRESS: Two-Trace Two-Dimensional (2T2D-COS) in the Analysis of Brain Tissue Sample Preparation Method.

Applied spectroscopy·2026
Same journal

EXPRESS: Simplified Protocol for Analyzing Polarization Properties of Scanning Tunneling Microscope (STM) Light Emission Spectra at an Oblique Angle.

Applied spectroscopy·2026
Same journal

EXPRESS: Monitoring a Polyurethane Synthesis by Fiber-Coupled Attenuated Total Reflection Fourier Transform Infrared Spectroscopy and Multivariate Curve Resolution-Alternating Least Squares.

Applied spectroscopy·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data
08:12

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data

Published on: February 16, 2024

Time-resolved diffuse optical spectroscopy: a differential absorption approach.

Paola Taroni1, Andrea Bassi, Lorenzo Spinelli

  • 1Dipartimento di Fisica, Politecnico di Milano, piazza Leonardo da Vinci 32, 20133 Milan, Italy. paola.taroni@fisi.polimi.it

Applied Spectroscopy
|November 16, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a new method for estimating spectral absorption changes in turbid media using time-resolved diffuse optical spectroscopy. The technique is robust, works regardless of sample size or shape, and is validated experimentally.

More Related Videos

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies
07:12

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies

Published on: November 19, 2020

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
09:25

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Published on: August 22, 2018

Related Experiment Videos

Last Updated: Jun 6, 2026

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data
08:12

An Introduction to Processing, Fitting, and Interpreting Transient Absorption Data

Published on: February 16, 2024

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies
07:12

Real-Time Monitoring of Neurocritical Patients with Diffuse Optical Spectroscopies

Published on: November 19, 2020

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
09:25

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Published on: August 22, 2018

Area of Science:

  • Biomedical Optics
  • Photonics
  • Spectroscopy

Background:

  • Turbid media characterization is crucial in various scientific fields.
  • Accurate estimation of spectral absorption properties is often challenging.
  • Diffuse optical spectroscopy (DOS) offers non-invasive optical measurement techniques.

Purpose of the Study:

  • To develop and validate a method for estimating spectral changes in absorption properties of turbid media.
  • To assess the impact of scattering and instrument response on the method's accuracy.
  • To demonstrate the practical applicability of the proposed technique.

Main Methods:

  • Utilizing time-resolved diffuse optical spectroscopy (TR-DOS).
  • Deriving the method from the Beer-Lambert law, assuming constant scattering.
  • Investigating spectral dependence of scattering and non-ideal instrument response theoretically and experimentally.

Main Results:

  • The developed method accurately estimates spectral absorption changes in turbid media.
  • The method is robust to moderate spectral dependence of scattering properties.
  • Experimental validation confirms the theoretical findings and practical utility.

Conclusions:

  • The proposed TR-DOS method provides a reliable approach for spectral absorption estimation.
  • The technique is applicable across various sample sizes and shapes.
  • This method offers a valuable tool for analyzing complex turbid media.